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p jak2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p jak2
    P Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+jak2/Phospho-Jak2+(Tyr1007%2F1008)+Rabbit+mAb/pm42815069-98-154-155
    Average 96 stars, based on 441 article reviews
    p jak2 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    other:

    Article Title: Danggui Buxue decoction ameliorates blood deficiency syndrome by suppressing IL-6/JAK2/STAT3 signaling pathway: An integrated Chinmedomics and bioinformatics study.
    Article Snippet: Ethnopharmacological relevance: Danggui Buxue Decoction (DBD) is a classic traditional Chinese medicine formula that has shown significant efficacy in treating blood deficiency syndrome (BDS), though its mechanisms of action

    Immunohistochemistry:

    Article Title: Retinoic acid receptor gamma (RARγ) drives M2-like macrophage polarisation via CFI to promote thyroid cancer progression.
    Article Snippet: .. The primary antibodies used in this investigation are listed below, along with the dilutions for immunohistochemistry (IHC) and Western blotting (WB) staining analysis: GAPDH (cat. 5174, CST), JAK2 (cat. IPB0510, Baijia), phospho- JAK2 (cat. 3771, CST), RARγ (cat. 8965, CST), STAT6 (cat. IPB6262, Baijia), phospho-STAT6 (9361, CST), CD206 (P80446R2, Abmart), β-actin (cat. 4970, CST), ARG1 (TD6657, abmart), Anti-C5a (ab281923, Abcam). .. Secondary antibodies for WB and IHC were horseradish peroxidase-conjugated goat anti-rabbit (cat. 12–348, SIGMA) or anti-mouse IgG (cat. 12–349, SIGMA).

    Western Blot:

    Article Title: Retinoic acid receptor gamma (RARγ) drives M2-like macrophage polarisation via CFI to promote thyroid cancer progression.
    Article Snippet: .. The primary antibodies used in this investigation are listed below, along with the dilutions for immunohistochemistry (IHC) and Western blotting (WB) staining analysis: GAPDH (cat. 5174, CST), JAK2 (cat. IPB0510, Baijia), phospho- JAK2 (cat. 3771, CST), RARγ (cat. 8965, CST), STAT6 (cat. IPB6262, Baijia), phospho-STAT6 (9361, CST), CD206 (P80446R2, Abmart), β-actin (cat. 4970, CST), ARG1 (TD6657, abmart), Anti-C5a (ab281923, Abcam). .. Secondary antibodies for WB and IHC were horseradish peroxidase-conjugated goat anti-rabbit (cat. 12–348, SIGMA) or anti-mouse IgG (cat. 12–349, SIGMA).

    Staining:

    Article Title: Retinoic acid receptor gamma (RARγ) drives M2-like macrophage polarisation via CFI to promote thyroid cancer progression.
    Article Snippet: .. The primary antibodies used in this investigation are listed below, along with the dilutions for immunohistochemistry (IHC) and Western blotting (WB) staining analysis: GAPDH (cat. 5174, CST), JAK2 (cat. IPB0510, Baijia), phospho- JAK2 (cat. 3771, CST), RARγ (cat. 8965, CST), STAT6 (cat. IPB6262, Baijia), phospho-STAT6 (9361, CST), CD206 (P80446R2, Abmart), β-actin (cat. 4970, CST), ARG1 (TD6657, abmart), Anti-C5a (ab281923, Abcam). .. Secondary antibodies for WB and IHC were horseradish peroxidase-conjugated goat anti-rabbit (cat. 12–348, SIGMA) or anti-mouse IgG (cat. 12–349, SIGMA).

    Cell-Signaling:

    Article Title: Two arabinans APRP70-1-1 and APRP70-1-2 from Angelica pubescens: structural characterization and immunomodulatory mechanism
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Incubation:

    Article Title: Two arabinans APRP70-1-1 and APRP70-1-2 from Angelica pubescens: structural characterization and immunomodulatory mechanism
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Produced:

    Article Title: Combining metabolic and checkpoint therapy: 1,25-dihydroxyvitamin D₃ enhances the antiviral efficacy of anti-PD-1 in BVDV infection.
    Article Snippet: Bovine viral diarrhea virus (BVDV) infection induces severe immunosuppression and peripheral blood lymphopenia, driven by PD-1/PD-L1-mediated T cell exhaustion.. Emerging evidence highlights 1,25-dihydroxyvitamin D3 (1,25(OH)2D3, VD), the active metabolite of vitamin D, as a critical modulator of antiviral immunity.. However, its interplay with PD-1 signaling in BVDV pathogenesis remains unexplored.

    Control:

    Article Title: Combining metabolic and checkpoint therapy: 1,25-dihydroxyvitamin D₃ enhances the antiviral efficacy of anti-PD-1 in BVDV infection.
    Article Snippet: Bovine viral diarrhea virus (BVDV) infection induces severe immunosuppression and peripheral blood lymphopenia, driven by PD-1/PD-L1-mediated T cell exhaustion.. Emerging evidence highlights 1,25-dihydroxyvitamin D3 (1,25(OH)2D3, VD), the active metabolite of vitamin D, as a critical modulator of antiviral immunity.. However, its interplay with PD-1 signaling in BVDV pathogenesis remains unexplored.



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    Effects of FUC and RO8191 on cartilage degeneration and <t>JAK2/STAT3</t> signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.
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    Effects of FUC and RO8191 on cartilage degeneration and <t>JAK2/STAT3</t> signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.
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    Effects of FUC and RO8191 on cartilage degeneration and <t>JAK2/STAT3</t> signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.
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    Effects of FUC and RO8191 on cartilage degeneration and <t>JAK2/STAT3</t> signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.
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    Effects of FUC and RO8191 on cartilage degeneration and <t>JAK2/STAT3</t> signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.
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    Effects of FUC and RO8191 on cartilage degeneration and <t>JAK2/STAT3</t> signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.
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    Image Search Results


    Effects of FUC and RO8191 on cartilage degeneration and JAK2/STAT3 signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.

    Journal: Molecular Medicine Reports

    Article Title: Fucoxanthin alleviates chondrocyte inflammation and osteoarthritis by modulating the JAK2/STAT3 signaling pathway

    doi: 10.3892/mmr.2026.13933

    Figure Lengend Snippet: Effects of FUC and RO8191 on cartilage degeneration and JAK2/STAT3 signaling in vivo . Representative (A) H&E-stained full-joint coronal sections and (B) corresponding high-magnification images of articular cartilage from ACLT rats treated with 200 mg/kg FUC, with or without RO8191 (n=6). Representative (C) Safranin O-Fast Green-stained full-joint coronal sections and (D) corresponding high-magnification images showing cartilage proteoglycan content (n=6). (E) OARSI scores of full-joint coronal sections, with corresponding high-magnification views (n=6). (F) Immunohistochemical staining and quantitative analysis of p-JAK2, total JAK2, p-STAT3 and total STAT3 in articular cartilage (n=6). Scale barrs: 200 µm for (A,C) full-joint coronal sections; 100 µm for (B,D) high-magnification sections. Data are presented as the mean ± SD. **P<0.01. ACLT, anterior cruciate ligament transection; FUC, fucoxanthin; OARSI, Osteoarthritis Research Society International; p, phosphorylated; JAK, Janus kinase.

    Article Snippet: The primary antibodies consisted of inducible nitric oxide synthase (iNOS; 1:1,000; cat. no. A14031; Abclonal Biotech Co., Ltd.), MMP3 (1:1,000; cat. no. A22328; ABclonal Biotech Co., Ltd.), MMP13 (1:1,000; cat. no. 18165-1; Proteintech Group, Inc.), cyclooxygenase-2 (COX-2; 1:1,000; cat. no. A1253; Abclonal Biotech Co., Ltd.), a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5; 1:1,000; cat. no. A2836; ABclonal Biotech Co., Ltd.), IL-6 (1:1,000; cat. no. CPA4914; Cohesion Biosciences), phosphorylated (p)-JAK2 (1:2,000; AP0531; cat. no. Abclonal Biotech Co., Ltd.), JAK2 (1:2,000; cat. no. A11497; Abclonal Biotech Co., Ltd.), β-actin (1:3,000; cat. no. GB11001; Wuhan Servicebio Technology Co., Ltd.), p-STAT3 (1:2,000; cat. no. T56566 ; Abmart Pharmaceutical Technology Co., Ltd.) and STAT3 (1:2,000; cat. no. T55292 ; Abmart Pharmaceutical Technology Co., Ltd.).

    Techniques: In Vivo, Staining, Immunohistochemical staining

    FUC reduces inflammatory responses associated with JAK2/STAT3 signaling in vitro . Primary rat chondrocytes were stimulated with IL-1β (10 ng/ml, 24 h) and treated with FUC (15 µM) and/or the JAK2 activator RO8191. (A) Western blot analysis and semi-quantitative evaluation of JAK2/STAT3 signaling components and inflammatory/catabolic protein expression (n=3). (B) Reverse transcription-quantitative PCR analysis of IL-6, MMP3, MMP13 and ADAMTS5 (n=3). (C) ELISA analysis of IL-6 secretion. (D) Immunofluorescence staining of MMP3 in chondrocytes with nuclei counterstained with DAPI, and corresponding quantitative fluorescence intensity analysis. Scale bar, 50 µm. Data are presented as the mean ± SD. *P<0.05; **P<0.01. NC, negative control; FUC, fucoxanthin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; iNOS, inducible nitric oxide synthase; p, phosphorylated; JAK, Janus kinase.

    Journal: Molecular Medicine Reports

    Article Title: Fucoxanthin alleviates chondrocyte inflammation and osteoarthritis by modulating the JAK2/STAT3 signaling pathway

    doi: 10.3892/mmr.2026.13933

    Figure Lengend Snippet: FUC reduces inflammatory responses associated with JAK2/STAT3 signaling in vitro . Primary rat chondrocytes were stimulated with IL-1β (10 ng/ml, 24 h) and treated with FUC (15 µM) and/or the JAK2 activator RO8191. (A) Western blot analysis and semi-quantitative evaluation of JAK2/STAT3 signaling components and inflammatory/catabolic protein expression (n=3). (B) Reverse transcription-quantitative PCR analysis of IL-6, MMP3, MMP13 and ADAMTS5 (n=3). (C) ELISA analysis of IL-6 secretion. (D) Immunofluorescence staining of MMP3 in chondrocytes with nuclei counterstained with DAPI, and corresponding quantitative fluorescence intensity analysis. Scale bar, 50 µm. Data are presented as the mean ± SD. *P<0.05; **P<0.01. NC, negative control; FUC, fucoxanthin; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; iNOS, inducible nitric oxide synthase; p, phosphorylated; JAK, Janus kinase.

    Article Snippet: The primary antibodies consisted of inducible nitric oxide synthase (iNOS; 1:1,000; cat. no. A14031; Abclonal Biotech Co., Ltd.), MMP3 (1:1,000; cat. no. A22328; ABclonal Biotech Co., Ltd.), MMP13 (1:1,000; cat. no. 18165-1; Proteintech Group, Inc.), cyclooxygenase-2 (COX-2; 1:1,000; cat. no. A1253; Abclonal Biotech Co., Ltd.), a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5; 1:1,000; cat. no. A2836; ABclonal Biotech Co., Ltd.), IL-6 (1:1,000; cat. no. CPA4914; Cohesion Biosciences), phosphorylated (p)-JAK2 (1:2,000; AP0531; cat. no. Abclonal Biotech Co., Ltd.), JAK2 (1:2,000; cat. no. A11497; Abclonal Biotech Co., Ltd.), β-actin (1:3,000; cat. no. GB11001; Wuhan Servicebio Technology Co., Ltd.), p-STAT3 (1:2,000; cat. no. T56566 ; Abmart Pharmaceutical Technology Co., Ltd.) and STAT3 (1:2,000; cat. no. T55292 ; Abmart Pharmaceutical Technology Co., Ltd.).

    Techniques: In Vitro, Western Blot, Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence, Negative Control